Coding

Part:BBa_K3726025:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-05)
Revision as of 16:59, 6 October 2021 by Fmjorge99 (Talk | contribs)


CDS_GarK (E. Coli BL21)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 355
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 355
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 355
    Illegal AgeI site found at 868
    Illegal AgeI site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been designed to be assemble within the part "BBa_K3726028" through Gibson Assembly.


Source

Coding sequence of this Glycerate 2-kinase has been obtained from Escherichia coli (strain BL21) genome thought PCR. Uniprot reference:https://www.uniprot.org/uniprot/P23524


References