Coding
mVenus-B3_

Part:BBa_K3781003

Designed by: Nicolas Bayer   Group: iGEM21_TU_Kaiserslautern   (2021-10-05)
Revision as of 18:19, 5 October 2021 by Nbayer (Talk | contribs)


mVenus, MoCloMania B3_B4

This part codes for the yellow fluorescent protein with the poetic name mVenus. It is a deviation of the famous green fluorescent protein GFP originally isolated from the bioluminescent jellyfish Aequorea victoria in 1962. By coupling a protein of interest with a fluorescent tag such as mVenus, the target protein can be easily and non-invasively detected within living cells as well as cell preparations with the help of fluorescence spectroscopy and microscopy.

SIZE 26.9 kDa

EXCITATION WAVELENGTH 515 nm

EMISSION WAVELENGTH 537 nm



No part name specified with partinfo tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 202
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 202
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 202
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 202
    Illegal NgoMIV site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/yfp
//function/reporter/fluorescence
Parameters
biologyyellow fluorescent protein
emission537 nm
excitation515 nm