Coding
mVenus-B3_
Part:BBa_K3781003
Designed by: Nicolas Bayer Group: iGEM21_TU_Kaiserslautern (2021-10-05)
mVenus, MoCloMania B3_B4
This part codes for the yellow fluorescent protein with the poetic name mVenus. It is a deviation of the famous green fluorescent protein GFP originally isolated from the bioluminescent jellyfish Aequorea victoria in 1962. By coupling a protein of interest with a fluorescent tag such as mVenus, the target protein can be easily and non-invasively detected within living cells as well as cell preparations with the help of fluorescence spectroscopy and microscopy.
SIZE 26.9 kDa
EXTINCTION WAVELENGTH 515 nm
EMISSION WAVELENGTH 537 nm
No part name specified with partinfo tag.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 202
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 202
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 202
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 202
Illegal NgoMIV site found at 679 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//cds/reporter/yfp
//function/reporter/fluorescence
//function/reporter/fluorescence
biology | yellow fluorescent protein |
emission | 537 nm |
excitation | 515 nm |