Coding

Part:BBa_K3726002:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-04)
Revision as of 13:23, 5 October 2021 by Sbarraga (Talk | contribs) (→‎Source)


CDS_Ccr


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 85
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 85
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 168
    Illegal BamHI site found at 507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 85
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 85
    Illegal NgoMIV site found at 763
    Illegal AgeI site found at 291
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of Ccr. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases



Source

Coding sequence of this Crotonyl-CoA reductase has been found within the genome of Streptomyces Collinus. Uniprot reference: https://www.uniprot.org/uniprot/Q53865

References

"Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true.