Plasmid
detection

Part:BBa_K3882001

Designed by: Fan Jiayu   Group: iGEM21_BS_United_China   (2021-09-07)
Revision as of 01:59, 3 October 2021 by YUNQI (Talk | contribs)


E. bsuahlscout-QS-bsuahlteminator

Part1 equipped with LuxR to sense and combine the autoinducer, N-Acyl Homoserine Lactone (AHL) in the environment. After that, LuxR-AHL complex will be activated and bind to luxr-AHL response QS element to initiate the expression of mcherry report gene. T7 promoter serves as starting the whole synthetic gene. Lac operator is used for releasing LuxR. LuxR functions as receiving AHL signal molecules, and activates mCherry red fluorescence gene. QS promoter is the place that LuxR can recruit RNA polymerase. mCherry is a red fluorescence reporting gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None