Composite

Part:BBa_K3905012:Design

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-13)
Revision as of 18:38, 13 September 2021 by Registry (Talk | contribs)

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T7 / gen3 210-3p-517-5p AND-Gate / Luciferase / Double Terminator / T7 / gen3 210-3p-517-5p AND-Gate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 108
    Illegal SapI.rc site found at 932


Design Notes

Had to ensure that only half the bases were bound with the complementary miRNA 517-5p and the other half would remain unbound to serve as a trigger. Needed to have a base trigger different to that of the anti-MiRNA 210-3p to ensure that these miRNAs wouldn't bind to each other. Had to ensure that the anti-Mirna alone wouldn't trigger the toehold switch.


Source

This is synthetic, designed in silico, using NUPACK.

References