Composite
Part:BBa_K3905008:Design
Designed by: Peter Heywood, Harry Weiniger Group: iGEM21_City_of_London_UK (2021-09-13)
T7 / gen2 517-5p Toehold Switch / Luciferase / Double Terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 41
Illegal XbaI site found at 79 - 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 41
Illegal XbaI site found at 79 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 41
Illegal XbaI site found at 79 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 922
Design Notes
We had to ensure that the toehold switch's sequence did not bind to the linker sequence deforming the structure, that the start codon was not exposed in the 'off' state and that the RBS held the correct formation in the 'on' state.
Source
This is synthetic, designed in silico.