Coding

Part:BBa_K3885008

Designed by: XIA YAO   Group: iGEM21_ZJUT-China   (2021-09-30)
Revision as of 05:05, 3 October 2021 by Sky1 (Talk | contribs)


ClpXP

ClpXP consists of two distinct proteins, a AAA+ ATPase called ClpX（BBa_K1911003） and a peptidase called ClpP（BBa_K1911002）. We incorporate the ClpX and ClpP to be a one-part. In protein substrates, ClpX recognizes unstructured peptide sequences (called tags or degrons), like ssrA--a specific C-terminal degradation tag, proceeds to unfold stable tertiary structure in the protein, and then spools or translocates the unfolded polypeptide chain into a sequestered proteolytic compartment in ClpP for degradation into small peptide fragments (Fig. 1). T--ZJUT-China--ClpXP.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
    Illegal BglII site found at 1628
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 387
    Illegal EcoRI site found at 1449
    Illegal EcoRI site found at 1794
    Illegal AgeI site found at 1110
    Illegal AgeI site found at 1848
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None