Coding
Part:BBa_K3799004
Designed by: Shubhamay Das,Lakshmi Prakash Group: iGEM21_IISER_Kolkata (2021-09-30)
Nisin PV, A synthetic antimicrobial peptide
This antimicrobial peptide is a synthetic variant of Nisin A produced naturally in Lactococcus lactis.Mutation of Serine to proline at position 29 (S29P) combined with an isoleucine to valine substitution at position 30 (I30V) helps in making Nisin PV resistant to nisin resistant protein which is expressed on the cell surface of certain pathogenic strains like Streptococcus agalactiae which can cleave Nisin A making it less active.Nisin Pv is not only resistant to NRP but also shows enhanced antimicrobial activity against S.uberis which is a main pathogen associated with Subclinical bovine mastitis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |