Plasmid

Part:BBa_K3731004:Design

Designed by: Hao Yin   Group: iGEM21_Nanjing-China   (2021-09-29)
Revision as of 04:49, 30 September 2021 by Registry (Talk | contribs)

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EPVM-PBBR1MCS-2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5166
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5166
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5166
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5166
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5166
    Illegal XbaI site found at 5181
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

For the construction of EPVM-PBBR1MCS-2, genomic DNA of E.Coli BL21 was used as the template to PCR-amplify ppk1, vgb and mazE with primers.

References