Composite

Part:BBa_K3905010:Design

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-13)
Revision as of 17:58, 13 September 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


T7 / gen3 210-3p-517-5p AND-Gate / Luciferase / Double Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 44
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 44
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 44
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 108
    Illegal SapI.rc site found at 932


Design Notes

Had to ensure that only half the bases were bound with the complementary miRNA 517-5p and the other half would remain unbound to serve as a trigger. Needed to have a base trigger different to that of the anti-MiRNA 210-3p to ensure that these miRNAs wouldn't bind to each other.


Source

This is synthetic, designed in silico, using NUPACK.

References