Coding
NphT7
Part:BBa_K3726000:Design
Designed by: Jorge Fernández Méndez Group: iGEM21_MADRID_UCM (2021-08-03)
CDS_NphT7
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 461
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614
Illegal NgoMIV site found at 105
Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 511
Illegal AgeI site found at 883 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part works as an strong RBS sequence specifically designed for Synechococcus elongatus PCC7942.
Source
The part has been artifically designed using DeNovoDNA software.