Coding
NphT7

Part:BBa_K3726000

Designed by: Jorge Fernández Méndez   Group: iGEM21_MADRID_UCM   (2021-08-03)
Revision as of 14:55, 3 August 2021 by Registry (Talk | contribs)

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CDS_NphT7

This is an synthetic RBS sequence specifically designed for achieving a high level of translation of the part BBa_KK3726000 , BBa_KK3726000 & BBa_KK3726000.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 461
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 511
    Illegal AgeI site found at 883
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None