Regulatory

Part:BBa_K3905005:Design

Designed by: Peter Heywood, Harry Weiniger   Group: iGEM21_City_of_London_UK   (2021-09-09)
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gen2 210-3p Toehold Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to ensure that the toehold switch's sequence did not bind to the linker sequence deforming the structure, that the start codon was not exposed in the 'off' state and that the RBS held the correct formation in the 'on' state.


Source

Synthetic. NUPACK

References