![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K116201
Designed by: Yuan-Hao Lee Group: iGEM08_NYMU-Taipei (2008-10-30)
ureD promoter from P mirabilis
An 328 bp promoter region, which can be assembled with urea binding proteins to activate the downstream gene expression.
pUreD is an inducible promoter that is activated by the UreR transcription factor in the presence of urea. pUreD is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [3].
pUreD is a bidirectional promoter driving transcription of the UreR and UreD protein subunits of the urease operon of P. mirabilis. pUreD contains two ribosome binding sites and a UreR binding site about upstream of the forward transcriptional start site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//rnap/prokaryote/pmirabilis
//direction/forward
//chassis/prokaryote/pmirabilis
//promoter
//regulation/positive
//direction/forward
//chassis/prokaryote/pmirabilis
//promoter
//regulation/positive
negative_regulators | |
positive_regulators | 1 |