Translational_Unit

Part:BBa_K3866017

Designed by: Ioanna Gkoni   Group: iGEM21_Thessaly   (2021-09-23)
Revision as of 20:07, 26 September 2021 by Igkoni (Talk | contribs)


PArtial T5 - CuO - MazF - double terminator

Fig.2:The level A of MazF toxin with the partial T5 promoter and the double terminator in an alpha1R vector.

Usage and Biology

This part includes the strong Partial T5 Promoter with the downstream operator Cuo. Also, it includes the toxin MazF that will be utilized to the toxin-antitoxin system and the double terminator.



Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector BBa_K3505009 and has overhangs compatible for GoldenBraid cloning.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

i

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Categories
Parameters
None