Coding
Part:BBa_K3804002
Designed by: iGEM21_UTokyo Group: iGEM21_UTokyo (2021-09-24)
Qsc receptor
This is a Qsc receptor which is designed to be used in S.Cerevisiae. QscR, homolog of lasR, binds with AHL and forms the AHL-QscR complex. Two AHL-QscR complexes form a dimer and activate pQsc. There are some receptors which bind with AHL, such as luxR and lasR. Originally, pQscR exists in Pseudomonas aeruginosa and is used for quorum sensing. In Pseudomonas aeruginosa, AHL expresses constitutively and the amount of AHL is related to the density of Pseudomonas aeruginosa. We create this part in order to detect Pseudomonas aeruginosa by using the part in yeast.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 103
Illegal PstI site found at 466 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 103
Illegal PstI site found at 466 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 103
Illegal XhoI site found at 25
Illegal XhoI site found at 76 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 103
Illegal PstI site found at 466 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 103
Illegal PstI site found at 466 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 241
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Categories
Parameters
None |