Coding
Part:BBa_K3832000
Designed by: Hongyu Chen,Dan Tan Group: iGEM21_XJTU-China (2021-09-26)
aroG (Mutant S211F)
This part encodes a mutant of AroG (3-deoxy-7-phosphoheptulonate synthase, EC 2.5.1.54 ), in which serine at 211 was replaced by phenylalanine. The mutant can relieve the allosteric inhibition of phenylalanine, thus increasing the catalytic rate and downstream product yield.
The enzyme that encoded by the sequence catalyze the following reactions: phosphoenolpyruvate+D-erythrose 4-phosphate+H2O =3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate +phosphate
Derived from E.coli DH5alpha strain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 934
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme/ligase
biology | E.coli |
kegg | EC 2.5.1.54 |
protein | aroG |