Composite

Part:BBa_K3715055

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-26)
Revision as of 14:08, 26 September 2021 by Registry (Talk | contribs)

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T7 promoter+RBS+Linker a+His+PETase_MT17+Linker b+T7 terminator

The part consists of T7 promoter,RBS and protein coding(Linker a+His+PETase_MT16+Linker b),T7 terminator and the biological module can be built into E.coil for protein expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 954
    Illegal XhoI site found at 968
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None