Coding

Part:BBa_K3715105

Designed by: Yuan Wang   Group: iGEM21_TJUSLS_China   (2021-09-25)
Revision as of 14:50, 25 September 2021 by Registry (Talk | contribs)

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T7 promoter+RBS+Linker a+His+Super1+Linker b+T7 terminator

PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbeIdeonella sakaiensis. Well, Super is a complicated mutation of PETase, which contains 11 mutation sites：S214H-I168R-W159H-S188Q-R280A-A180I-G165A-Q119Y-L117F-T140D-I208F.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 863
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 878
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 863
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 863
    Illegal NgoMIV site found at 138
    Illegal NgoMIV site found at 192
    Illegal NgoMIV site found at 219
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None