Composite

Part:BBa_K3832001

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-09-26)
Revision as of 06:28, 26 September 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Induced expression circuit of aroG (Mutant s211f)

This Part is the Induced expression circuit of CDS aroG (Mutant s211f) (BBa_K3832000).It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.

Our team use GoldenGate assembly to assemble the subparts into this circuit. This parts contains the BioBrick prefix and at both ends.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2554
    Illegal NheI site found at 2577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2541
    Illegal XhoI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 453


[edit]
Categories
//cds/enzyme/ligase
Parameters
biologyE.coli
keggEC 2.5.1.54
proteinaroG