Coding
Part:BBa_K3715001:Design
Designed by: Yuan Wang Group: iGEM21_TJUSLS_China (2021-09-25)
Super1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 783
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 783
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 783
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 783
Illegal NgoMIV site found at 58
Illegal NgoMIV site found at 112
Illegal NgoMIV site found at 139 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We know this patr from a novel(https://dx.doi.org/10.1021/acscatal.0c05126). DuraPETase is another wild type as well as PETase.
Source
Recently, a novel devised a computational strategy (greedy accumulated strategy for protein engineering, GRAPE) to improve the robustness of a PETase fromIdeonella sakaiensis.