Part:BBa_K4035008

Dimerization of the copper metallothionein 1 : CUP1-GGGGS(EAAAK)2GGGGS-CUP1

This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a semi-rigid linker made of the GGGGS(EAAAK)2GGGGS amino acid sequence.

Usage and Biology

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035014). The linker is composed of two flexible regions GGGGS separated by two rigid regions EAAAK, making the linker rigid in the center with 2 flexible arms attached to the CUP1 proteins.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 391

biology	CUP1
chassis	S.cerevisiae
control	pCTcon2V5, WT EBY100
direction	Forward
function	copper binding protein