Coding

Part:BBa_K4035007

Designed by: Anissa Hammi   Group: iGEM21_EPFL   (2021-09-10)
Revision as of 09:33, 10 September 2021 by Registry (Talk | contribs)

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Dimerization of the copper metallothionein 1 : CUP1-GGGGSEAAAKGGGGS-CUP1

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to improve the copper fixation efficiency in yeast, CUP1 was linked to a second sequence of the same protein. The linker is a semi-rigid linker and is made of the GGGGSEAAAKGGGGS amino acid sequences. GGGGS is flexible and EAAAK is rigid, making the linker rigid in the center with 2 flexible arms attached to the CUP1 proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 190
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds/membrane/extracellular
//chassis/eukaryote/yeast
Parameters
biologyCUP1
chassisS.cerevisiae
controlpCTcon2V5, WT EBY100
directionForward
functioncopper binding protein