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Part:BBa_J70027:Design

Designed by: Tom Knight   Group: Knight Lab   (2008-11-04)
Revision as of 18:37, 4 November 2008 by Tk (Talk | contribs) (→‎Design Notes)

Mesoplasma florum plasmid origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 372


Design Notes

No native restriction sites. Added biobrick ends.

The gene for PE contains no CGG codons.

The primer names are for J70023 -- this is a shortened version

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2

GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R

The start of J70027 matches the origin start in the pSci1 plasmid.

Source

Genbank AJ969070

References