Measurement

Part:BBa_K3971010:Design

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-08-12)
Revision as of 08:29, 12 August 2021 by Registry (Talk | contribs)

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cscB (sucrose permease) transport efficiency characterization with different promoters.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2764
    Illegal PstI site found at 362
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2577
    Illegal PstI site found at 362
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1103
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2764
    Illegal PstI site found at 362
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2764
    Illegal PstI site found at 362
    Illegal NgoMIV site found at 1589
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1633
    Illegal BsaI.rc site found at 152


Design Notes

gfs


Source

The terminator (BBa_B0015) is available in the iGEM distribution kit. PcpcB-m6 was generously donated by the Wangikar Lab at IIT Bombay. The restriction enzyme sites are incorporated into the cassette by adding them in primers. The homology arms for Neutral Site 1 can be amplified from the S. elongatus UTEX 2973 genome.

References