Coding

Part:BBa_K3971002

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-08-09)
Revision as of 16:46, 9 August 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


cscA - Sucrose-6-phosphate hydrolase (Invertase) from E.coli W.

cscA allows E.coli to grow on sucrose as a sole carbon source. Knockout strains of cscA could not grow on sucrose suggesting that it is essential for sucrose metabolism. Intracellular sucrose is hydrolyzed to glucose and fructose and it its expression is derepressed at low sucrose concentrations (0.2 %). Overexpression of cscA alone has been shown to confer sucrose utilizing ability in different E.coli strains, however with low growth rates. [1] Overexpression has also been shown to result in leakage of the invertase, which was identified in the extracellular medium [2]. However, in wild-type strains, invertase activities are exclusively intracellular [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 255
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None