Part:BBa_K3971001

cscK - D-Fructokinase from E.coli W

Usage and Biology

cscK is a part of the csc operon found in E.coli W which allows for sucrose consumption and metabolism. The cscK gene product phosphorylates fructose, which then enters glycolysis directly [1]. At low sucrose concentrations, the csc operon is repressed. Removal of cscK or cscR (the csc operon's repressor) was shown to be involved in derepression and their combinatorial knockout led to an improved growth rate in low sucrose concentrations. [2]

csc operon in E.coli W for sucrose metabolism. Figure adapted from: Bruschi, Michele, et al. "A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains." Biotechnology advances 30.5 (2012): 1001-1010.

E. coli W with a deletion of cscK could grow on 2% sucrose, with no compromise on the growth rate, and it was determined that the gene is not entirely essential for sucrose utilization and that there might be an alternate path for fructose utilization at low sucrose concentrations (0.2%) [2].

References

1: Bruschi, Michele, et al. "A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains." Biotechnology advances 30.5 (2012): 1001-1010.

2: Sabri, Suriana, Lars K. Nielsen, and Claudia E. Vickers. "Molecular control of sucrose utilization in Escherichia coli W, an efficient sucrose-utilizing strain." Applied and environmental microbiology 79.2 (2013): 478-487.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 39
  Illegal BglII site found at 483
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]