Coding

Part:BBa_K1486000

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-12)
Revision as of 00:31, 18 October 2014 by Tsimonet (Talk | contribs)

CpxR coding region

CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 61
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 472
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//direction/forward
regulator
transcriptional
Parameters
chassisE coli
directionForward
functionTransciption regulator
negative_regulatorsCpxA (phosphatase activity)
positive_regulatorsCpxA (Histidine kinase activity when activated)
rbsElowitz
resistanceChloramphenicol