Part:BBa_K1500994
Dam
Involved in methylating DNA, overexpression causes 6.4 * 10^-4 /// 6.4 * 10^4 mutation rate increase, mostly catalyzing transversions.
Information contributed by City of London UK (2021)
Part information is collated here to help future users of the BioBrick registry.
dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1]
It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3]
Furthermore, it may also be involved in DNA replication [4]
Reaction
a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine
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