Part:BBa_K3767003

3-24 ScFv sequence linked to Green Fluorescent Protein (GFP)

The 3-24 ScFv sequence is composed of light chains (VL) and heavy chains (VH) from antibodies that are linked by a peptide linker (1). The sequence was made to detect the bacteria that result in Lyme Disease, known as Borrelia Burgdorferi using the OspA. The design of the 3-24 ScFv sequence was based on past research done by Ghosh and Huber to optimize its binding affinity to both the OspA and E. coli expression (2). The green fluorescent protein (GFP) is a reporter gene that is derived from the jellyfish Aequeora victoria. It can be used as a marker for gene expression and protein targeting in intact cells due to its extremely visible fluorescence (3). This part is being registered as a composite of a previously registered sequence (BBa_K3767000) and GFP (BBa_E0040).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1379