Part:BBa_K3767003
3-24 ScFv sequence linked to Green Fluorescent Protein (GFP)
Alkaline phosphatase coding sequence (no rbs, promoter, or terminator present) from Citrobacter. This bacterium's phoA was chosen due simply to the absense of Biobrick restriction sites. There may be some neutral point mutations relative to the stated sequence, but the construct is fully functional. This part exists in plasmid pSB1A2 (pSB1A2-Bca1032), and in J61003 (pBca1020-Bca1032) under the control of a Tet promoter and RBS b0034.
The green fluorescent protein (GFP) gene can be used as reporter gene and express in Starmerella bombocola.
3-24 ScFv Sequence: GAAGTCCAGTTGGTACAGAGTGGCGCTGAGGTAAAAAAACCGGGAGCAAGTGTTAAAGTCTCTTGCAAAGCCTCGGGTTACACCTTTACAGATTATTATCTCCATTGGGTCCGTCAGGCGCCGGGCCAAGGACTTGAGTGGTTAGGTCGAATTAACCCATCATCCGGTGCAACTTACAGCCCGCAGCGCTTCCAGGGCCGTGTTACTATGACCACTGACACGTCCATATCCACCGCCTATATGGAATTAAGTAGCCTGCGTAGCGACGATACCGCCGTGTACTTTTGTGCCACGCTGACGACCTTTAACATCTGGGCTTTTGACTACTGGGGCCAGGGTACCCTGGTGTCATCTGGCGGTGGTGGGTCGGGCGGTGGTGGAAGCGGCGGCGGCGGATCAAGCGATATTCAAATGACCCAGTCGCCATCGTCTCTGTCCGCGAGCGTGGGCGATCGCGTGACCATTACATGCCGGGCGTCGCAAAGCATCAGTACATATCTGAATTGGTATCAACAGAAACCTGGTAAGGCACCCAAACTGCTGATCTTCACAGCGTCATCGCTTCAATCTGGGGTTCCGAGCACGTTCAGTGGGAGTGGGTCCGGTACTGATTTCACCTTGACGATCAGCTCTCTACAGCCGGAAGATTTCGCTACCTATTACTGTCAGCAGAGCTATTCAGCGACGTTTACGTTTGGCGGGGGCACCAAAGTGGAAATTAAGCGC
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1379
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