Composite

Part:BBa_K3767003

Designed by: Cecilia Hernandez-Hosaka & Griffin Watson-Boehnisch   Group: iGEM21_Queens_Canada   (2021-06-05)
Revision as of 17:29, 5 June 2021 by Registry (Talk | contribs)

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3-24 ScFv sequence linked to Green Fluorescent Protein (GFP)

Alkaline phosphatase coding sequence (no rbs, promoter, or terminator present) from Citrobacter. This bacterium's phoA was chosen due simply to the absense of Biobrick restriction sites. There may be some neutral point mutations relative to the stated sequence, but the construct is fully functional. This part exists in plasmid pSB1A2 (pSB1A2-Bca1032), and in J61003 (pBca1020-Bca1032) under the control of a Tet promoter and RBS b0034.

The green fluorescent protein (GFP) gene can be used as reporter gene and express in Starmerella bombocola.

3-24 ScFv Sequence: GAAGTCCAGTTGGTACAGAGTGGCGCTGAGGTAAAAAAACCGGGAGCAAGTGTTAAAGTCTCTTGCAAAGCCTCGGGTTACACCTTTACAGATTATTATCTCCATTGGGTCCGTCAGGCGCCGGGCCAAGGACTTGAGTGGTTAGGTCGAATTAACCCATCATCCGGTGCAACTTACAGCCCGCAGCGCTTCCAGGGCCGTGTTACTATGACCACTGACACGTCCATATCCACCGCCTATATGGAATTAAGTAGCCTGCGTAGCGACGATACCGCCGTGTACTTTTGTGCCACGCTGACGACCTTTAACATCTGGGCTTTTGACTACTGGGGCCAGGGTACCCTGGTGTCATCTGGCGGTGGTGGGTCGGGCGGTGGTGGAAGCGGCGGCGGCGGATCAAGCGATATTCAAATGACCCAGTCGCCATCGTCTCTGTCCGCGAGCGTGGGCGATCGCGTGACCATTACATGCCGGGCGTCGCAAAGCATCAGTACATATCTGAATTGGTATCAACAGAAACCTGGTAAGGCACCCAAACTGCTGATCTTCACAGCGTCATCGCTTCAATCTGGGGTTCCGAGCACGTTCAGTGGGAGTGGGTCCGGTACTGATTTCACCTTGACGATCAGCTCTCTACAGCCGGAAGATTTCGCTACCTATTACTGTCAGCAGAGCTATTCAGCGACGTTTACGTTTGGCGGGGGCACCAAAGTGGAAATTAAGCGC


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379


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