Generator
phoA

Part:BBa_K3767001

Designed by: Griffin Watson-Boehnisch   Group: iGEM21_Queens_Canada   (2021-06-08)
Revision as of 16:14, 8 June 2021 by GriffinWB (Talk | contribs)


Alkaline Phosphatase optimized for E. Coli w/ 40x catalytic activity

Alkaline phosphatases are a group of isoenzymes that catalyze the hydrolysis of organic phosphate esters present in the extracellular space (1) [1] . This catalytic activity is commonly used in diagnostic tests as the cleavage of the phosphate ester produces fluorescence visible to the naked eye.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biologyEscherichia coli
proteinphoA