Coding

Part:BBa_K3657013

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 03:34, 28 October 2020 by JuliaLanger (Talk | contribs)


RNA binding Pentatricopeptide Repeat Protein - Motif GUAGAG


This Part is an example of a PPR Protein, assembled with the PPR Domains that are specific for the four RNA bases.

Adenine:BBa_K3657006

Guanine:BBa_K3657007

Uracil:BBa_K3657008

Cytosine:BBa_K3657009

It is an OFF target PPR Protein for plasmides, containing the RBS BBa_B0032 because it binds to the RNA sequence GUAGAG, which is not found in the RBS. It is an ON target PPR Protein the broccoli aptamer because it binds to the RNA sequence ACACAG, which can be find in the loop or stem region of the broccoli aptamer.
For more information, visit iGEM Heidelberg 2020

Usage and Biology

PPRs are originally found in Arabidopsis thaliana and can bind RNA. In chloroplasts, they take part in many post-translational regulation processes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None