Part:BBa_K3657012

RNA binding Pentatricopeptide Repeat Protein - Motif ACACAG

This Part is an example of a PPR Protein, assembled with the PPR Domains that are specific for the four RNA bases.

Adenine:BBa_K3657006

Guanine:BBa_K3657007

Uracil:BBa_K3657008

Cytosine:BBa_K3657009

It is an ON target PPR Protein for plasmides, containing the RBS BBa_B0032 because it binds to the RNA sequence ACACAG, which is part of the RBS. It is an OFF target PPR Protein for the broccoli aptamer because it binds to the RNA sequence ACACAG, which cannot be found in the broccoli aptamers.
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Usage and Biology

PPRs are originally found in Arabidopsis thaliana and can bind RNA. In chloroplasts, they take part in many post-translational regulation processes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterisation

In combination with part <a href="https://parts.igem.org/Part:BBa_K3657012">BBa_K3657012</a>, an assay was conducted, messuaring