Composite
pTRE-GF-SL
Part:BBa_K3502011
Designed by: Jionglin Chen Group: iGEM20_SYSU-CHINA (2020-10-27)
a GFP-STEM-LOOP-apoptin vector controlled by TRE-on system
This year, we tested the editing efficiency of ADAR1 by adding a pTRE system and a stem ring. The essence grains were added with fluorescent protein after the PTRE promoter to report the editing efficiency. We found that the addition of the stem ring inhibited the background leak of the PTRE promoter and had no significant effect on its peak value, which we thought might be an important pTRE improvement.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1348
Illegal BamHI site found at 10 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1015
[edit]
Categories
Parameters
None |