Composite
pTRE-GF-SL

Part:BBa_K3502011

Designed by: Jionglin Chen   Group: iGEM20_SYSU-CHINA   (2020-10-27)
Revision as of 02:49, 28 October 2020 by Registry (Talk | contribs)

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a GFP-STEM-LOOP-apoptin vector controlled by TRE-on system

This year, we tested the editing efficiency of ADAR1 by adding a pTRE system and a stem ring. The essence grains were added with fluorescent protein after the PTRE promoter to report the editing efficiency. We found that the addition of the stem ring inhibited the background leak of the PTRE promoter and had no significant effect on its peak value, which we thought might be an important pTRE improvement.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1348
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1015


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