Coding

Part:BBa_K1531004

Designed by: Patrik D   Group: iGEM14_SF_Bay_Area_DIYbio   (2014-10-13)
Revision as of 02:58, 28 October 2020 by This-is-Min (Talk | contribs)


Bovine beta casein (B variant) with full alpha-factor yeast secretion signal

Beta casein is one of four major caseins (cheese proteins) found in bovine cheese. Together with Kappa casein, it is necessary and sufficient to create casein micelles.

At least 12 different genetic variants of bovine Beta casein have been identified and characterized. The B allele used here is associated with improved milk coagulation.

The coding sequence for this gene is preceded by a secretion signal derived from the yeast mating pheromone alpha-factor in Saccharomyces cerevisiae, facilitates secretion of heterologous proteins in yeast. We used a full length form of the full alpha-factor protein with kex and ste13 protease cleavage sites (FAKS). (www.pnas.org/content/81/15/4642.short). In addition, the entire construct is flanked by SapI restriction sites, for cloning into DNA2.0's Electra system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1
    Illegal SapI.rc site found at 910


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Categories
Parameters
None