Composite

Part:BBa_K3606082

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)
Revision as of 02:27, 28 October 2020 by Zhenru-Zhang (Talk | contribs)


PlacIq_lacI_Ptac driven DsbA-CaAP

PlacIq_lacI_Ptac driven OmpA-CaAP is a part of our signal-peptide guided CaAP secretion system. <p>To test the secretion efficiency of our 5 secretion peptides fused CaAP(Calcium Absorption Peptide) guided by 5 secretion peptides, we put them under the regulation of the iPTG inducible promoter, Ptac, and expressed them in E.coli. BL21 strain respectively. Thus, quantified characterization of these secretion peptides fused with CaAP is expected to be achieved through the different quantity of the target protein, namely CaAP, in the bacterial lysate and the culture medium.

The 5 signal peptides candidates for CaAP secretion are listed below.

Signal Peptide Number for Registry
NSP4 BBa_K3606042
OmpA BBa_K3606043
DsbA BBa_K3606030
PelB BBa_K208004
PhoA BBa_K808028

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1408
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None