Coding

Part:BBa_K3392000

Designed by: Xian-en Zhang   Group: iGEM20_UCAS-China   (2020-10-23)
Revision as of 17:15, 23 October 2020 by Registry (Talk | contribs)

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Doc-SsrA

Doc is a protein usually used in bacteria toxin-antitoxin system (TA system). This TA system is derived from the bacteriophage P1, which lysogenizes in Escherichia coli cells as a stable low-copy plasmid. Doc plays a role of toxin. SsrA is a degradation tag of 11-residue carboxy-terminal peptide tail to the incomplete nascent chain. Doc-SsrA is a part connects Doc and SsrA with TEV site. When there is no TEV in the cell, toxin protein will be degraded; after TEV is produced, it can function normally. In our biosafety switch, this design will reduce the loss of plasmid due to the leaky expression, thus reducing the escape rate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None