Composite

Part:BBa_K3588014

Designed by: Peter Heywood, Louis Gringras   Group: iGEM20_CLS_CLSG_UK   (2020-10-24)
Revision as of 15:50, 24 October 2020 by Registry (Talk | contribs)

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MazE-MazF Hypoxia induced kill switch

MazF is under control of the promoter BBa_K1720002, upregulating in anoxic conditions. MazE is under control of the constitutive promoter BBa_K747096, downregulating in anoxic conditions.

In anoxic conditions, there should be excess MazF, leading to apoptotic cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1043
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BamHI site found at 655
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1043
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety
//biosafety/kill_switch
Parameters
None