Regulatory

Part:BBa_K3425103

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-08)
Revision as of 09:44, 8 October 2020 by Registry (Talk | contribs)

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DBD-CaFF construct (pSB3K3)

Part BBa_K3425100 expressed on pSB3K3 with J23100 promoter and B0034 RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2729
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2729
    Illegal NheI site found at 1384
    Illegal NheI site found at 2757
    Illegal NheI site found at 2780
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2729
    Illegal BglII site found at 2853
    Illegal BglII site found at 3727
    Illegal XhoI site found at 177
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2729
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2729
    Illegal XbaI site found at 2744
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 1470
    Illegal AgeI site found at 1793
    Illegal AgeI site found at 3419
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2498


[edit]
Categories
Parameters
None