Composite

Part:BBa_K3610051:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-09)
Revision as of 22:12, 16 October 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


CORE ectodomain / SmallBit NanoLuc for S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1740
    Illegal BamHI site found at 373
    Illegal BamHI site found at 1765
    Illegal BamHI site found at 2117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Chosing an appropriate way to link the CORE ectodomain with the SmallBit NanoLuc sequence required a short sequence with small amino acids that allowes in frame translation. The flag tag is necessary for western blotting.

Source

BBa_K2809002, BBa_K3610010, BBa_M10019, BBa_K416001, BBa_K3610016

References

England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.

Wang, Lei; Albert, Markus; Einig, Elias; Fürst, Ursula; Krust, Damaris; Felix, Georg (2016): The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein. In: Nature plants 2, S. 16185. DOI: 10.1038/nplants.2016.185.