Composite

Part:BBa_K3606121

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)
Revision as of 00:33, 28 October 2020 by LX2020 (Talk | contribs)


P14 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR

Usage and Biology:

This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P14 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5306
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5263
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5306
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5306
    Illegal PstI site found at 2327
    Illegal PstI site found at 2360
    Illegal NgoMIV site found at 1989
    Illegal AgeI site found at 2161
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None