Part:BBa_K3331005

nodA(MazF)

ndoA (ydcD, mazF) [ Bacillus subtilis subsp. subtilis str. 168 ]

Encodes EndoA, an endoribonuclease that inactivates cellular mRNAs by cleaving them at specific, but frequently occurring sites. EndoA has similar cleavage specificity to MazF and PemK and yields cleavage products with 3′‐phosphate and 5′‐hydroxyl groups, typical of EDTA‐resistant degradative RNases.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

In the figure, the bacteria in this plate contained plasmids of pBAD-MazF. Three of 6-1 groups were the control group without arabinose induction, and three of 6-4 groups were the experimental group with 1.5 M/L arabinose induction. Where, the dilution factor of the group with * is 10^4, and the dilution factor of the group without * is 10^6.

Figure 1: Experimental medium. 6-1 is blank control ,6-4 is experimental group.