Coding

Part:BBa_K3657002

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-20)
Revision as of 00:04, 28 October 2020 by JuliaLanger (Talk | contribs)


RNA binding Pumilio Homology Domain (Uracil)

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides. This part is the Uracil binding Pumby Domain. Combined with the other Pumby Domains (BBa_K3657001

, BBa_K3657002

, BBa_K3657003

) it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: BBa_K3657004

, BBa_K3657005

)

Usage and Biology

Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 70
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None