Reporter

Part:BBa_K3657017:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 22:12, 27 October 2020 by Liza ch (Talk | contribs) (→‎Source)


Broccoli Aptamer with added GUAGAG RNA Loop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed based on the original Broccoli aptamer with the use of DesignSimple in order to exclude the BsmbI cutting site and introduce the GUAGAG loop.

Due to the COVID pandemic we were not able to experimentally prove that this Broccoli modification binds DFHBI correctly in vitro.

Source

Synthetic

References