Device

Part:BBa_K1682018:Experience

Designed by: Nikola Dyulgyarov   Group: iGEM15_HKUST-Rice   (2015-09-17)
Revision as of 22:48, 27 October 2020 by Saifkhan (Talk | contribs) (→‎Applications of BBa_K1682018)


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Background - Lambert_GA 2020

Background - Lambert_GA 2020

To optimize functionality, Lambert_GA altered the original part designed by HKUST-Rice 2015 by replacing the original terminators with rrNB T1 terminator and T7Te terminator after super folder GFP and a rrnBT1 T1 terminator after TetR. Nar Operon Background Native to E. coli, the Nar Operon regulates anaerobic gene expression in response to two electron acceptors: nitrate and nitrite. This system consists of two homologous membrane-bound sensor proteins (NarX and NarQ) as well as two homologous DNA-binding response regulators (NarL and NarP). NarL’s conjugate is the membrane-bound NarX protein while NarP’s conjugate is the membrane-bound NarQ protein. Our team is utilizing this system to test nitrate and nitrite levels, NarL and NarP expression, and downstream GFP expression through mathematical models.

<img src="T--Lambert_GA--_Nardiagram.png"

UNIQece6db09f9aec016-partinfo-00000000-QINU UNIQece6db09f9aec016-partinfo-00000001-QINU