Coding

Part:BBa_K3454043

Designed by: Zhiyu Fang   Group: iGEM20_ShanghaiTech_China   (2020-10-27)
Revision as of 20:46, 27 October 2020 by Registry (Talk | contribs)

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AsCas12a_RVRR_6xHis_MBP

This is the coding sequence of AsCas12a mutant E174R/S542R/K548V/N552R with 6x His and MBP tags.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1393
    Illegal PstI site found at 4864
    Illegal PstI site found at 5062
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2083
    Illegal PstI site found at 1393
    Illegal PstI site found at 4864
    Illegal PstI site found at 5062
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
    Illegal BglII site found at 5052
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1393
    Illegal PstI site found at 4864
    Illegal PstI site found at 5062
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1393
    Illegal PstI site found at 4864
    Illegal PstI site found at 5062
    Illegal NgoMIV site found at 4596
    Illegal AgeI site found at 3493
    Illegal AgeI site found at 3964
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79
    Illegal BsaI.rc site found at 2236
    Illegal BsaI.rc site found at 3898
    Illegal SapI.rc site found at 1486


[edit]
Categories
Parameters
None