Coding

Part:BBa_K3629011

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 22:16, 27 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Usage and Biology)


Nourseothricin resistance gene

Nourseothricin acetyltransferase (nat1) gene from Streptomyces noursei.

Usage and Biology

This is the coding sequence for nourseothricin resistance, which is a robust selection marker that can be used in many eukaryotes, specifically yeast, including Yarrowia lipolytica -the chassis for our project. Particularly for Y. lipolytica there are not many available auxotrophy-based selection markers due to the limited number of auxotrophic strains publically available for use. Therefore, antibiotic resistance genes are a suitable alternative. To use this antibiotic 50-200µg/mL is added to media depending on the organism.

The part is a key part of our collection and functions as a destination vector for other Y. lipolytica parts when cloned into a plasmid with a promoter and terminator sequence attached.

Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
//awards/part_collection
//cds/selectionmarker/antibioticresistance
//chassis/eukaryote/yeast
Parameters
None