Coding

Part:BBa_K142201:Design

Designed by: Reine Byun   Group: iGEM08_ETH_Zurich   (2008-10-28)
Revision as of 04:08, 30 October 2008 by Rbyun (Talk | contribs) (→‎Design Notes)

T4 ligase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 332


Design Notes

The sequence was optimised to remove all Biobrick restriction sites.

Source

source

References